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KMID : 0381920060360040173
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Korean Journal of Microscopy
2006 Volume.36 No. 4 p.173 ~ p.183
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The Liver Protecting Effect of Pomegranate (Punica granatum) Seed Oil in Mice Treated with CCl4
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Kim Dong-Hee
Lee Young-Mi
Yoon Yang-Suk
Kwon Ki-Rok
Park Dae-Bokd
Park Seung-Kyu
Lee Kyu-Jae
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Abstract
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Recently, the pomegranate seed oil (PSO) has been reported to have various effects including anti-cancer
effect. In this study, we examined the liver-protecting effect of the PSO on the hepatotoxicity induced by CCl4 using the BALB/c mice. The PSO was made from dried seeds of black pomegranate (Punica grantum) by heating and squeezing. The expreimental animals were divided into 3 groups; control group treated with olive oil only, experimental group 1 treated with CCl4 only, and experimental group 2 treated with PSO and CCl4. 24 hours after injection of CCl4 into the peritoneal cavity, we collected the blood samples to measure the level of serological factors; aspartate minotransferase (AST), alanine aminotransferase (ALT), total protein, albumin, total bilirubin, direct bilirubin and alkaline phosphatase. Simultaneously we observed the histological change of liver under the light and electron microscope.
As the result, AST and ALT showed 88.7±14.9 IU/L and 22.0±3.12 IU/L in the control group, 1963.7± 1212.9 IU/L and 4495.4±2803.6 IU/L in the experimental group 1, and 432.2±260.1 IU/L and 692.3±433.1 IU/L in the experimental group 2. The experimental group 2 showed significant difference as compared with experimental group 1 (P?0.005). In histological study, the experimental group 2 was recovered than experimental group 1 which had abnormal mitochondria, increase of lysosomes, and severe necrosis at the central vein zones. These results indicated that the PSO had the liver protecting effect. However, The further study on the relationship between ingredients of pomegranate seed and liver protecting effect is in need.
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KEYWORD
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Protecting effect, Pomegranate, Seed oil, Liver, CCl4
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